Coding

Part:BBa_K1097000:Design

Designed by: Xiaofei Song   Group: iGEM13_CAU_China   (2013-09-17)

adh2（Saccharomyces cerevisiae）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No considerations are taken since no intron exist in this gene when constructing this part. But during protein expression, you may consider codon optimization.

You simply insert this part into an expression vector like pET-28a(+) (Novagen), and the corresponding enzyme yeast ADH2 can be expressed under suitable condition. This enzyme preferentially catalyzes the conversion of ethanol to acetaldehyde and can act on a variety of primary unbranched aliphatic alcohols.

Source

Our part is the PCR product of yeast adh2 using genomic DNA as template.

References

Leskovac V, Trivic S,and Pericin D (2002). The three zinc-containing alcohol dehydrogenases from baker's yeast, Saccharomyces cerevisiae. FEMS Yeast Res. 2: 481-494.

NCBI_ID:NM_001182812.1 http://www.ncbi.nlm.nih.gov/nuccore/NM_001182812.1